Biophysical and biochemical investigations of RNA catalysis in the hammerhead ribozyme.

5. The three-dimensional structure of the hammerhead ribozyme 262 5.1 Enzyme–inhibitor complexes 262 5.2 Enzyme–substrate complex in the initial state 264 5.3 Hammerhead ribozyme self-cleavage in the crystal 264 5.4 The requirement for a conformational change 265 5.5 Capture of conformational intermediates using crystallographic freeze-trapping 266 5.6 The structure of a hammerhead ribozyme ‘early ’ conformational intermediate 267 5.7 The structure of a hammerhead ribozyme ‘ later ’ conformational intermediate 268 5.8 Is the conformational change pH dependent ? 269 5.9 Isolating the structure of the cleavage product 271 5.10 Evidence for and against additional large-scale conformation changes 274 5.11 NMR spectroscopic studies of the hammerhead ribozyme 278